MELK ELISA Kit (Rat) (OKCD09080)

Catalog No: OKCD09080

Size:96WELLS

MELK ELISA Kit (Rat) (OKCD09080) Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Maternal Embryonic Leucine Zipper Kinase (MELK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Maternal Embryonic Leucine Zipper Kinase (MELK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Maternal Embryonic Leucine Zipper Kinase (MELK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Maternal Embryonic Leucine Zipper Kinase (MELK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Maternal Embryonic Leucine Zipper Kinase (MELK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Maternal Embryonic Leucine Zipper Kinase (MELK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MELK Microplate: 96 Wells (12 x 8 Well strips)
  • MELK Lyophilized Standard: 2
  • 100X MELK HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.063ng/mL

Target Info

• Gene Symbol: MELK
• Gene Full Name: maternal embryonic leucine zipper kinase
• Alias Symbols: hPK38; Protein kinase Eg3; Tyrosine-protein kinase MELK
• NCBI Gene Id: 362510
• Protein Name: maternal embryonic leucine zipper kinase
• Uniprot ID: D4A6T6
• Protein Accession #: NP_001102132.1
• Nucleotide Accession #: NM_001108662.1