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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for MELK Antibody (OAEB01902) |
|---|
| Predicted Species Reactivity | Bovine|Dog|Human|Mouse |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| Additional Information | Reactivity Info: Tested: Human; Expected: Human, Mouse, Dog, Cow |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | MELK |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 91%; Rabbit: 100%; Rat: 91% |
| Peptide Sequence | KRLVEDILSSCKV |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1ug/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3ug/ml. |
| Reference | * Lin ML, Park JH, Nishidate T, Nakamura Y, Katagiri T.; Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family.; Breast Cancer Res. 2007;9(1):R17.; Pubmed 17280616 * Nagase T, Seki N, Ishikawa K, Tanaka A, Nomura N.; Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1.; DNA Res 1996 Feb 29;3(1):17-24; Pubmed 8724849 |
| Gene Symbol | MELK |
|---|---|
| Gene Full Name | maternal embryonic leucine zipper kinase |
| Alias Symbols | HPK38;maternal embryonic leucine zipper kinase;pEg3 kinase;protein kinase Eg3;protein kinase PK38;tyrosine-protein kinase MELK. |
| NCBI Gene Id | 9833 |
| Protein Name | Maternal embryonic leucine zipper kinase |
| Uniprot ID | Q14680 |
| Protein Accession # | NP_055606.1 |
| Molecular Weight | 74.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MELK Antibody (OAEB01902)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse".
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How long will it take to receive "MELK Antibody (OAEB01902)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MELK Antibody (OAEB01902)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MELK Antibody (OAEB01902)"?
This target may also be called "HPK38;maternal embryonic leucine zipper kinase;pEg3 kinase;protein kinase Eg3;protein kinase PK38;tyrosine-protein kinase MELK." in publications.
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What is the shipping cost for "MELK Antibody (OAEB01902)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MELK Antibody (OAEB01902)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MELK Antibody (OAEB01902)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "74.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MELK Antibody (OAEB01902)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MELK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MELK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MELK"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MELK"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MELK"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MELK"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
