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Catalog No: OAPC00211 (Formerly GWB-24ECBF)
Size:100 ul
Price: $390.00
SKU
OAPC00211
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for OAPC00211
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Cow, Dog
Product FormatLiquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
ClonalityPolyclonal
HostRabbit
ApplicationWB
Additional InformationPackaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and StorageFor long term storage -20C is recommended. Stable at -20C for at least 1 year.
ImmunogenPhosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK 1.
PurificationPrepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Predicted Homology Based on Immunogen SequenceCow; Dog; Human; Mouse; Rat
SpecificitySpecific for the ~45k MEK 1 protein phosphorylated at Thr292 in Western blots.
Application InfoThe antibody has been directly tested for reactivity in Western blots with recombinant MEK1. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primates and rat based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Western blot of recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.
ReferenceAdams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163.
Ahn NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209.
Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.
Crews CM, Alessandrini A, Erikson RL (1992) The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258:478-480.
Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266:10043-10046.
Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG (1994) Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J Biochem (Tokyo) 116:304-314.
Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos-phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88:6142-6146.
Gene SymbolMAP2K1
Alias SymbolsMEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1
NCBI Gene Id5604
Protein NameDual specificity mitogen-activated protein kinase kinase 1
Description of TargetMEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).
Uniprot IDQ02750
Protein Accession #NP_002746
Nucleotide Accession #NM_002755
Molecular Weight~45kDa
  1. What is the species homology for "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog".

  2. How long will it take to receive "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)" provided in?

    This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    This target may also be called "MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1" in publications.

  5. What is the shipping cost for "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "~45kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "MEK 1 Antibody (Phospho-Thr29) (OAPC00211)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "MAP2K1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "MAP2K1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "MAP2K1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "MAP2K1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "MAP2K1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "MAP2K1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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