MEK 1/2 Antibody (Bi-Phospho-Ser218/222) (OAPC00210)

Catalog No: OAPC00210 (Formerly GWB-1D1D45)

Size:100 ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: For long term storage -20C is recommended. Stable at -20C for at least 1 year.
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.
• Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Homology Based on Immunogen Sequence: Cow; Dog; Human; Mouse; Rat; Zebrafish
• Specificity: Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
• Application Info: The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, non-human primates, rat, Xenopus and zebra fish based on the fact that these species have 100% homology in the amino acid sequence used as antigen for this antibody.
  
• Reference: Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163.
  Ahn NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209.
  Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.
  Crews CM, Alessandrini A, Erikson RL (1992) The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258:478-480.
  Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266:10043-10046.
  Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos-phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88:6142-6146.

Target Info

• Gene Symbol: MAP2K1/2
• Alias Symbols: MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1
• NCBI Gene Id: 5604
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• Description of Target: MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
• Uniprot ID: Q02750
• Protein Accession #: NP_002746
• Nucleotide Accession #: NM_002755
• Molecular Weight: ~45kDa