Catalog No: OCOA05318
Size:20UG
Price: $179.00
SKU
OCOA05318
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MEIS2 Protein Lysate (OCOA05318)

Datasheets/ManualsPrintable datasheet for MEIS2 Protein Lysate (OCOA05318)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMEIS2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMEIS2
Gene Full NameMeis homeobox 2
Alias SymbolsCPCMR;homeobox protein Meis2;HsT18361;Meis homolog 2;Meis1, myeloid ecotropic viral integration site 1 homolog 2;Meis1-related gene 1;meis1-related protein 1;MRG1;TALE homeobox protein Meis2.
NCBI Gene Id4212
Protein NameA0A024R9L4, B3KPD8, NM_170676, NP_733776, O14770
Description of TargetcDNA FLJ31654 fis, clone NT2RI2004230, highly similar to Homeobox protein Meis2, Homeobox protein Meis2, homeobox protein Meis2 isoform d [Homo sapiens], Homo sapiens Meis homeobox 2 (MEIS2), transcript variant d, mRNA, Meis1, myeloid ecotropic viral integration site 1 homolog 2 (Mouse), isoform CRA_b
Uniprot IDhttps://www.uniprot.org/uniprot/O14770
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_733776
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_170676
Molecular Weight51.1 kDa
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