Catalog No: OCOA21388
Size:20UG
Price: $169.00
SKU
OCOA21388
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

MEF2C Protein Lysate (OCOA21388)

Datasheets/ManualsPrintable datasheet for MEF2C Protein Lysate (OCOA21388)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMEF2C HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMEF2C
Gene Full Namemyocyte enhancer factor 2C
Alias SymbolsC5DELq14.3;DEL5q14.3;MADS box transcription enhancer factor 2, polypeptide C;Myocyte enhancer factor 2C;myocyte-specific enhancer factor 2C;NEDHSIL.
NCBI Gene Id4208
Protein NameNM_001193348, NP_001180277, Q06413
Description of TargetHomo sapiens myocyte enhancer factor 2C (MEF2C), transcript variant 4, mRNA, Myocyte-specific enhancer factor 2C, myocyte-specific enhancer factor 2C isoform 4 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q06413
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001180277
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001193348
Molecular Weight45.4 kDa
Write Your Own Review
You're reviewing:MEF2C Protein Lysate (OCOA21388)
Your Rating