MEF2A Antibody (Phospho-Ser408) (OAAF00013)

Catalog No: OAAF00013 (Formerly GWB-ASB019)

Size:100 ug

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using MEF2A (Phospho-Ser408) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEF2A (Phospho-Ser408) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using MEF2A (Phospho-Ser408) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEF2A around the phosphorylation site of Ser408.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MEF2A (phospho-Ser408) antibody detects endogenous levels of MEF2A only when phosphorylated at serine 408.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~3000; IHC: 1:50~100; IF: 1:100~1:500; ELISA: 1:40000
• Reference: Cyril Rauch, Am J Physiol Cell Physiol, Mar 2005; 288: C593 - C605.
  OI Ornatsky, Nucleic Acids Res., Jul 1999; 27: 2646 - 2654.
  David P. Sparling, Am J Physiol Endocrinol Metab, Apr 2007; 292: E1149 - E1156.
  Eric N. Olson Sci, Aging Knowl. Env

Target Info

• Gene Symbol: MEF2A
• Gene Full Name: myocyte enhancer factor 2A
• Alias Symbols: ADCAD1;MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A);mef2;myocyte-specific enhancer factor 2A;RSRFC4;RSRFC9;serum response factor-like protein 1.
• NCBI Gene Id: 4205
• Protein Name: Myocyte-specific enhancer factor 2A
• Description of Target: Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.
• Uniprot ID: Q02078
• Protein Accession #: NP_001124398.1
• Molecular Weight: 54 kDa