MED31 ELISA Kit (Mouse) (OKEH05648)

Catalog No: OKEH05648

Size:96T

Mouse MED31 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Med31 ELISA Kit (Mouse) (OKEH05648) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Med31 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Med31 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Med31 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 92.1%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <7.9%, n = 20
  Mean Inter-Assay Precision - <9.6%, n = 20
• ELISA Kit Component:
  • MED31 Microplate: 96 Wells (12 x 8 Well strips)
  • MED31 Lyophilized Standard: 2
  • 100X Biotinylated MED31 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.395 ng/mL
• Specificity: Natural and recombinant Mouse Mediator of RNA polymerase II transcription subunit 31
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Med31
• Gene Full Name: mediator complex subunit 31
• Alias Symbols: 3110004H13Rik;CGI-125;l11Jus;l11Jus15;Mediator complex subunit 31;mediator complex subunit SOH1;mediator of RNA polymerase II transcription subunit 31;mediator of RNA polymerase II transcription, subunit 31 homolog.
• NCBI Gene Id: 67279
• Protein Name: Q9CXU1
• Description of Target: Mediator of RNA polymerase II transcription subunit 31
• Uniprot ID: https://www.uniprot.org/uniprot/Q9CXU1
• Protein Accession #: NP_080344.2
• Nucleotide Accession #: NM_026068.2
• Protein Size (# AA): 131