MED31 ELISA Kit (Bovine) (OKEH07739)

Catalog No: OKEH07739

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MED31 ELISA Kit (Bovine) (OKEH07739) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MED31 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MED31 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MED31 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.7% (n = 20)
  Mean Inter-assay CV%: <=11.9% (n = 20)
• ELISA Kit Component:
  • MED31 Microplate: 96 Wells (12 x 8 Well strips)
  • MED31 Lyophilized Standard: 2
  • 100X Biotinylated MED31 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  Subcellular Location: Nucleus
  Unigene: Bt.88324
  SMR: A0JNN3
  String: 9913.ENSBTAP00000029148
  KEGG: bta:532868
  Pfam: PF05669
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Mediator of RNA polymerase II transcription subunit 31
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MED31
• Gene Full Name: mediator complex subunit 31
• Alias Symbols: Mediator complex subunit 31;mediator complex subunit SOH1;mediator of RNA polymerase II transcription subunit 31.
• NCBI Gene Id: 532868
• Protein Name: A0JNN3
• Description of Target: Mediator of RNA polymerase II transcription subunit 31
• Uniprot ID: https://www.uniprot.org/uniprot/A0JNN3
• Protein Accession #: NP_001071457.1
• Nucleotide Accession #: NM_001077989.2