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Catalog No: ARP50698_P050-HRP
Size:100ul
Price: $434.00
SKU
ARP50698_P050-HRP
Availability: Domestic: within 1-2 days delivery | International: 1-2 days
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MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)

Datasheets/ManualsPrintable datasheet for anti-MED25 (ARP50698_P050-HRP) antibody
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Zebrafish
Product FormatLiquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
ClonalityPolyclonal
HostRabbit
ConjugationHRP: Horseradish Peroxidase
ApplicationWB, CHIP
Reconstitution and StorageAll conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
ImmunogenThe immunogen is a synthetic peptide directed towards the N terminal region of human MED25
PurificationAffinity Purified
Predicted Homology Based on Immunogen SequenceCow: 100%; Dog: 100%; Guinea Pig: 85%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100%
Peptide SequenceSynthetic peptide located within the following region: EGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYV
Concentration0.5 mg/ml
Blocking PeptideFor anti-MED25 (ARP50698_P050-HRP) antibody is Catalog # AAP50698 (Previous Catalog # AAPP44777)
Subunit25
Publications

Sela, D. et al. Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6-alpha. J. Biol. Chem. 288, 26179-87 (2013). WB, Bovine, Human, Rat, Dog, Zebrafish, Pig, Mouse, Guinea pig 23864652

Gene SymbolMED25
Gene Full NameMediator complex subunit 25
Alias SymbolsP78, ACID1, ARC92, BVSYS, PTOV2, CMT2B2, TCBAP0758
NCBI Gene Id81857
Protein NameMediator of RNA polymerase II transcription subunit 25
Description of TargetThis gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Uniprot IDQ71SY5
Protein Accession #NP_112235
Nucleotide Accession #NM_030973
Protein Size (# AA)747
Molecular Weight78kDa
Protein InteractionsUBC; MED19; MED26; EPAS1; NOTCH1; DREB2A; CTDP1; MED6; THRA; RXRA; RARA; MED1; NR3C1; ESR1; CREBBP; SREBF1; MED25; MED15; MED16; MED13; MED12; MED7; MED17; MED23; MED14; CDK8; ZC3H13; QKI; TRIP4; TADA2A; MED28; OBFC1; TRRAP; UL48; MED9; MED8; HCFC1; MED10
  1. What is the species homology for "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Zebrafish".

  2. How long will it take to receive "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".

  3. What buffer format is "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)" provided in?

    This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    This target may also be called "P78, ACID1, ARC92, BVSYS, PTOV2, CMT2B2, TCBAP0758" in publications.

  5. What is the shipping cost for "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "78kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "MED25 Antibody - N-terminal region : HRP (ARP50698_P050-HRP)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "MED25"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "MED25"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "MED25"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "MED25"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "MED25"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "MED25"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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