MED14 Antibody (OAAF02032)

Catalog No: OAAF02032 (Formerly GWB-ASD305)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED14 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of COS7 cells, using MED14 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MED14.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MED14 antibody detects endogenous levels of total MED14 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Yoshikawa H., Genomics 49:237-246(1998).
  Ryu S., Nature 397:446-450(1999).
  Rachez C., Nature 398:824-828(1999).

Target Info

• Gene Symbol: MED14
• Gene Full Name: mediator complex subunit 14
• Alias Symbols: activator-recruited cofactor 150 kDa component;ARC150;Cofactor required for Sp1 transcriptional activation subunit 2;cofactor required for Sp1 transcriptional activation, subunit 2 (150kD);CRSP complex subunit 2;CRSP150;CRSP2;CSRP;CXorf4;DRIP150;EXLM1;hRGR1;human homolog of yeast RGR1;Mediator complex subunit 14;mediator of RNA polymerase II transcription subunit 14;RGR1;RGR1 homolog;thyroid hormone receptor-associated protein complex 170 kDa component;thyroid hormone receptor-associated protein complex component TRAP170;transcriptional co-activator CRSP150;Transcriptional coactivator CRSP150;TRAP170;vitamin D receptor-interacting protein complex component DRIP150;vitamin D3 receptor-interacting protein complex 150 kDa component.
• NCBI Gene Id: 9282
• Protein Name: Mediator of RNA polymerase II transcription subunit 14
• Description of Target: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
• Uniprot ID: O60244
• Protein Accession #: NP_004220.2
• Molecular Weight: 160 kDa