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Datasheets/Manuals | Printable datasheet for MED12 Antibody (OAAF02597) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human MED12. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | MED12 antibody detects endogenous levels of total MED12 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:5000 |
Reference | Ito M., Mol. Cell 3:361-370(1999). Nagase T., DNA Res. 3:17-24(1996). Philibert R.A., Mol. Psychiatry 3:303-309(1998). |
Gene Symbol | MED12 |
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Gene Full Name | mediator complex subunit 12 |
Alias Symbols | activator-recruited cofactor 240 kDa component;ARC240;CAG repeat protein 45;CAGH45;FGS1;HOPA;human opposite paired;Kohtalo homolog;Kto;MED12S;Mediator complex subunit 12;mediator of RNA polymerase II transcription subunit 12;mediator of RNA polymerase II transcription, subunit 12 homolog;OHDOX;OKS;OPA1;OPA-containing protein;putative mediator subunit 12;thyroid hormone receptor-associated protein complex 230 kDa component;thyroid hormone receptor-associated protein, 230 kDa subunit;TNRC11;TRAP230;trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit);trinucleotide repeat-containing gene 11 protein. |
NCBI Gene Id | 9968 |
Protein Name | Mediator of RNA polymerase II transcription subunit 12 |
Description of Target | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. |
Uniprot ID | Q93074 |
Protein Accession # | NP_005111.2 |
Molecular Weight | 247 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MED12 Antibody (OAAF02597)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "MED12 Antibody (OAAF02597)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MED12 Antibody (OAAF02597)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MED12 Antibody (OAAF02597)"?
This target may also be called "activator-recruited cofactor 240 kDa component;ARC240;CAG repeat protein 45;CAGH45;FGS1;HOPA;human opposite paired;Kohtalo homolog;Kto;MED12S;Mediator complex subunit 12;mediator of RNA polymerase II transcription subunit 12;mediator of RNA polymerase II transcription, subunit 12 homolog;OHDOX;OKS;OPA1;OPA-containing protein;putative mediator subunit 12;thyroid hormone receptor-associated protein complex 230 kDa component;thyroid hormone receptor-associated protein, 230 kDa subunit;TNRC11;TRAP230;trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit);trinucleotide repeat-containing gene 11 protein." in publications.
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What is the shipping cost for "MED12 Antibody (OAAF02597)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MED12 Antibody (OAAF02597)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MED12 Antibody (OAAF02597)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "247 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MED12 Antibody (OAAF02597)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MED12"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MED12"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MED12"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MED12"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MED12"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MED12"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.