MED1 Antibody (OAAF02458)

Catalog No: OAAF02458 (Formerly GWB-ASD687)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using MED1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MED1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MED1 antibody detects endogenous levels of total MED1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:10000
• Reference: Drane P., Oncogene 15:3013-3024(1997).
  Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
  Naeaer A.M., Nature 398:828-832(1999).

Target Info

• Gene Symbol: MED1
• Gene Full Name: mediator complex subunit 1
• Alias Symbols: activator-recruited cofactor 205 kDa component;ARC205;CRSP1;CRSP200;DRIP205;DRIP230;Mediator complex subunit 1;mediator of RNA polymerase II transcription subunit 1;p53 regulatory protein RB18A;PBP;peroxisome proliferator-activated receptor-binding protein;PPAR-binding protein;PPARBP;PPARG binding protein;PPARGBP;RB18A;thyroid hormone receptor-associated protein complex 220 kDa component;thyroid hormone receptor-associated protein complex component TRAP220;thyroid receptor interacting protein 2;Thyroid receptor-interacting protein 2;TRAP220;TR-interacting protein 2;TRIP2;TRIP-2;vitamin D receptor-interacting protein 230 kD;vitamin D receptor-interacting protein complex component DRIP205.
• NCBI Gene Id: 5469
• Protein Name: Mediator of RNA polymerase II transcription subunit 1
• Description of Target: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
• Uniprot ID: Q15648
• Protein Accession #: NP_004765.2
• Molecular Weight: 168 kDa