Catalog No: OPCD00125
Price: $0.00
SKU
OPCD00125
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for MECP2 Recombinant Protein (OPCD00125) |
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Predicted Species Reactivity | Homo sapiens|Human |
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Product Format | Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
Host | E.coli |
Conjugation | Unconjugated |
Application | Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot |
Additional Information | Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method) Residues: Met1-Lys177 Predicted Isoelectric Point: 9.6 Subcellular Location: Nucleus |
:: | Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. |
Reconstitution and Storage | 2°C to 8°C|-80°C |
Concentration | 200 ug/mL (prior to lyoph) |
Purity | > 90% |
Source | E.coli |
Tag | N-terminal His Tag |
Gene Symbol | MECP2 |
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Gene Full Name | methyl-CpG binding protein 2 |
Alias Symbols | AUTSX3;meCp-2 protein;methyl-CpG-binding protein 2;MRX16;MRX79;MRXS13;MRXSL;PPMX;RS;RTS;RTT. |
NCBI Gene Id | 4204 |
Protein Name | Methyl-CpG-binding protein 2 |
Description of Target | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
Uniprot ID | P51608 |
Protein Accession # | NP_001104262.1 |
Nucleotide Accession # | NM_001110792.1 |
Protein Size (# AA) | Met1~Lys177 |
Molecular Weight | 30kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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