Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OCOA12841
Size:20UG
Price: $179.00
SKU
OCOA12841
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

MECP2 Protein Lysate (OCOA12841)

Datasheets/ManualsPrintable datasheet for MECP2 Protein Lysate (OCOA12841)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMECP2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMECP2
Gene Full Namemethyl-CpG binding protein 2
Alias SymbolsAUTSX3;meCp-2 protein;methyl-CpG-binding protein 2;MRX16;MRX79;MRXS13;MRXSL;PPMX;RS;RTS;RTT.
NCBI Gene Id4204
Protein NameD3YJ43, NM_004992, NP_004983, P51608
Description of TargetHomo sapiens methyl-CpG binding protein 2 (MECP2), transcript variant 1, mRNA, Methyl-CpG-binding protein 2, methyl-CpG-binding protein 2 isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P51608
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_004983
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_004992
Molecular Weight52.3 kDa
Write Your Own Review
You're reviewing:MECP2 Protein Lysate (OCOA12841)
Your Rating