MECP2 ELISA Kit (Human) (OKEH08204)

Catalog No: OKEH08204

Size:96T

Human MECP2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MECP2 ELISA Kit (Human) (OKEH08204) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MECP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MECP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MECP2 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.0% (n = 20)
  Mean Inter-assay CV%: <=10.0% (n = 20)
• ELISA Kit Component:
  • MECP2 Microplate: 96 Wells (12 x 8 Well strips)
  • MECP2 Lyophilized Standard: 2
  • 100X Biotinylated MECP2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
  Subcellular Location: Nucleus Colocalized with methyl-CpG in the genome.
  Unigene: Hs.200716
  SMR: P51608
  String: 9606.ENSP00000395535
  KEGG: hsa:4204
  MIM: 105830
  Pfam: PF01429
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 10.3pg/mL
• Specificity: Natural and recombinant Human Methyl-CpG-binding protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MECP2
• Gene Full Name: methyl-CpG binding protein 2
• Alias Symbols: AUTSX3;meCp-2 protein;methyl-CpG-binding protein 2;MRX16;MRX79;MRXS13;MRXSL;PPMX;RS;RTS;RTT.
• NCBI Gene Id: 4204
• Protein Name: P51608
• Description of Target: Methyl-CpG-binding protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/P51608
• Protein Accession #: NP_001104262.1
• Nucleotide Accession #: NM_001110792.1