MeCP2 Antibody - C-terminal region (OAAB15949)

Catalog No: OAAB15949

Size:400ul

Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (C413) (Cat. # OAAB15949). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.

MeCP2 Antibody (C-term) (Cat. # OAAB15949) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: LiquidPBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC, ELISA
• Additional Information: Cellular Location: Nucleus. Note=Colocalized with methyl-CpG in the genome.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-428 amino acids from the C-terminal region of human MeCP2.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: MECP2
• Alias Symbols: MECP2, Methyl-CpG-binding protein 2
• Protein Name: Methyl-CpG-binding protein 2
• Description of Target: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
• Uniprot ID: Q95LG8
• Molecular Weight: 52 kDa