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Datasheets/Manuals | Printable datasheet for anti-MDM4 (AVARP06007_P050-HRP) antibody |
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Predicted Species Reactivity | Human, Cow, Dog, Horse, Pig, Rabbit |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human MDM4 |
Predicted Homology Based on Immunogen Sequence | Cow: 80%; Dog: 93%; Horse: 93%; Human: 100%; Pig: 86%; Rabbit: 80% |
Peptide Sequence | Synthetic peptide located within the following region: IPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLA |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-MDM4 (AVARP06007_P050-HRP) antibody is Catalog # AAPS31602 |
Reference | Danovi,D., et al., (2004) Mol. Cell. Biol. 24 (13), 5835-5843 |
Publications | Schlaeger, C. et al. Etiology-dependent molecular mechanisms in human hepatocarcinogenesis. Hepatology 47, 511-20 (2008). WB, Bovine, Dog, Horse, Human, Pig, Rabbit 18161050 |
Gene Symbol | MDM4 |
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Gene Full Name | Mdm4 p53 binding protein homolog (mouse) |
Alias Symbols | HDMX, MDMX, MRP1, BMFS6 |
NCBI Gene Id | 4194 |
Protein Name | Protein Mdm4 |
Description of Target | MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Uniprot ID | O15151 |
Protein Accession # | NP_002384 |
Nucleotide Accession # | NM_002393 |
Protein Size (# AA) | 490 |
Molecular Weight | 55kDa |
Protein Interactions | RNF115; CAPN7; CD160; MDM2; TP53; UBC; MDM4; USP2; YWHAG; UBE2D2; PRKAA1; BCL2; RPS20; RPS15; RPL37; YWHAQ; HSP90AA1; CSNK1A1; PPP1CC; PPP1CB; PPP1CA; RB1; APP; UBE2D3; UBE2D1; MNAT1; ABL1; LRSAM1; TRIM55; TRIM46; MKRN3; YWHAZ; YWHAE; YWHAB; CDKN1A; RFWD2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Cow, Dog, Horse, Pig, Rabbit".
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How long will it take to receive "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
This target may also be called "HDMX, MDMX, MRP1, BMFS6" in publications.
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What is the shipping cost for "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "55kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MDM4 Antibody - N-terminal region : HRP (AVARP06007_P050-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MDM4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MDM4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MDM4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MDM4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MDM4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MDM4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.