MDM4 Antibody - N-terminal region (AVARP06007_T100)

Catalog No: AVARP06007_T100

WB Suggested Anti-MDM4 Antibody
Titration: 2.5 ug/ml
Positive Control: HepG2 Whole Cell

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Cow, Dog, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MDM4
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 80%; Dog: 93%; Horse: 93%; Human: 100%; Pig: 86%; Rabbit: 80%
• Peptide Sequence: Synthetic peptide located within the following region: MLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRT
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-MDM4 (AVARP06007_T100) antibody is Catalog # AAP30507 (Previous Catalog # AAPP01143)

Target Info

• Reference: Gilkes,D.M., (2008) Mol. Cell. Biol. 28 (6), 1999-2010
• Gene Symbol: MDM4
• Gene Full Name: Mdm4 p53 binding protein homolog (mouse)
• Alias Symbols: HDMX, MDMX, MRP1, BMFS6
• NCBI Gene Id: 4194
• Protein Name: Protein Mdm4
• Description of Target: MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions.The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: O15151
• Protein Accession #: NP_002384
• Nucleotide Accession #: NM_002393
• Protein Size (# AA): 490
• Molecular Weight: 55kDa
• Protein Interactions: RNF115; CAPN7; CD160; MDM2; TP53; UBC; MDM4; USP2; YWHAG; UBE2D2; PRKAA1; BCL2; RPS20; RPS15; RPL37; YWHAQ; HSP90AA1; CSNK1A1; PPP1CC; PPP1CB; PPP1CA; RB1; APP; UBE2D3; UBE2D1; MNAT1; ABL1; LRSAM1; TRIM55; TRIM46; MKRN3; YWHAZ; YWHAE; YWHAB; CDKN1A; RFWD2