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MDM4 antibody - N-terminal region (AVARP06007_P050)

100 ul
$289.00
In Stock

Conjugation Options

AVARP06007_P050-FITC Conjugated

AVARP06007_P050-HRP Conjugated

AVARP06007_P050-Biotin Conjugated

Gene Symbol:
MDM4
NCBI Gene Id:
4194
Official Gene Full Name:
Mdm4 p53 binding protein homolog (mouse)
Protein Name:
Protein Mdm4
Swissprot Id:
O15151
Protein Accession #:
NP_002384
Nucleotide Accession #:
NM_002393
Alias Symbols:
DKFZp781B1423, MDMX, MGC132766, MRP1, HDMX
Replacement Item:
This antibody may replace item sc-14738 from Santa Cruz Biotechnology.
Description of Target:
MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Size (# AA):
490
Molecular Weight:
55kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MDM4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MDM4.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human MDM4
Species Reactivity:
Cow, Dog, Horse, Human, Pig, Rabbit
Predicted Homology Based on Immunogen Sequence:
Cow: 80%; Dog: 93%; Horse: 93%; Human: 100%; Pig: 86%; Rabbit: 80%
Complete computational species homology data:
Anti-MDM4 (AVARP06007_P050)
Peptide Sequence:
Synthetic peptide located within the following region: IPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLA
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
RNF115; CAPN7; CD160; MDM2; TP53; UBC; MDM4; USP2; YWHAG; UBE2D2; PRKAA1; BCL2; RPS20; RPS15; RPL37; YWHAQ; HSP90AA1; CSNK1A1; PPP1CC; PPP1CB; PPP1CA; RB1; APP; UBE2D3; UBE2D1; MNAT1; ABL1; LRSAM1; TRIM55; TRIM46; MKRN3; YWHAZ; YWHAE; YWHAB; CDKN1A; RFWD2
Blocking Peptide:
For anti-MDM4 (AVARP06007_P050) antibody is Catalog # AAPS31602
Datasheets/Manuals:
Printable datasheet for anti-MDM4 (AVARP06007_P050) antibody
Target Reference:
Danovi,D., et al., (2004) Mol. Cell. Biol. 24 (13), 5835-5843
Publications:

Schlaeger, C. et al. Etiology-dependent molecular mechanisms in human hepatocarcinogenesis. Hepatology 47, 511-20 (2008). WB, Cow, Dog, Horse, Human, Pig, Rabbit 18161050

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02/01/2017 16:24
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Product Review: MDM4 antibody - N-terminal region (AVARP06007_P050) in 143B cell using Western blot

Product page for MDM4 antibody - N-terminal region (AVARP06007_P050)


Researcher: Luis C. Anton, PhD, Centro de Biologia Molecular Severo Ochoa

Application: Western blotting

Species + Tissue/Cell type: Lane 1: 20ug 143B cell lysate

Primary antibody dilution: 1:1000

Secondary antibody: Anti-rabbit-HRP

Secondary antibody dilution: 1:10000




Questionnaire:
How do Aviva's reagents play a role in your experimental goals? Evaluation of the effect of a drug on MDM4's stability
How would you rate this antibody on a scale from 1-5 (5=best) and why? 4. MDM4 band is clearly visible above background, although requires long incubation times with the Ab (best if overnight)
Would you use this antibody in future experiments? Yes
Have you used another antibody which has worked in your application? No
Do you believe the information about the reagent on Aviva's website is correct? Yes
How did you store the antibody after re-suspension? Aliquots at -20 degree C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): Human osteosarcoma cell line 143B; 20 ug cell lysate
How many different experimental trials were conducted using the antibody sample? Three different settings.
How was this sample prepared? Cell pellet boiled in SDS-PAGE sample buffer.
Primary antibody dilution and incubation time: 1:1000 / 3 hrs
Secondary antibody used and dilution and incubation time: HRP/anti-Rabbit IgG from Southern Biotech 1/10000
Please include your detailed WB Procedure/Protocol here: Transferred overnight in tank system. Only worked with PVDF in our hands; did not work in one experimento using notrocellulose.
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