- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-MDM2 (OAGA01316) antibody |
---|
Predicted Species Reactivity | Human, Mouse |
---|---|
Product Format | Liquid 1XPBS, 20% glycerol (pH 7) with 0.025% Proclin300 as a preservative. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | CHIP, IHC-FFPE, IP, WB |
:: | Positive Controls: Jurkat, Raji, NCl-H929, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, MDM2-transfected 293T, HepG2, HepG2 (starvation for 16 hr, and 1 uM Staurosporine treatment for 24 hr) Cellular Localization: Nucleus, nucleoplasm, cytoplasm, nucleolus |
Reconstitution and Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Immunogen | Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Predicted Homology Based on Immunogen Sequence | Rat (81%), Dog (91%), Cat (95%), Pig (93%), Rhesus Monkey (98%), Bovine (95%) |
Concentration | 1.2 mg/ml |
Application Info | IHC-FFPE: 1:100~1000 IP: 1:100~500 WB: 1:500~3000 |
Gene Symbol | MDM2 |
---|---|
Gene Full Name | MDM2 proto-oncogene, E3 ubiquitin protein ligase |
Alias Symbols | HDMX, LSKB, hdm2, ACTFS |
NCBI Gene Id | 4193 |
Description of Target | This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. |
Uniprot ID | Q00987 |
Molecular Weight | 55 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "MDM2 Antibody (OAGA01316)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
-
How long will it take to receive "MDM2 Antibody (OAGA01316)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "MDM2 Antibody (OAGA01316)" provided in?
This item is provided in "Liquid 1XPBS, 20% glycerol (pH 7) with 0.025% Proclin300 as a preservative.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MDM2 Antibody (OAGA01316)"?
This target may also be called "HDMX, LSKB, hdm2, ACTFS" in publications.
-
What is the shipping cost for "MDM2 Antibody (OAGA01316)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "MDM2 Antibody (OAGA01316)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "MDM2 Antibody (OAGA01316)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "55 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MDM2 Antibody (OAGA01316)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "MDM2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "MDM2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "MDM2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "MDM2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "MDM2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "MDM2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.