MDH2 Antibody (OAGA00438)

Catalog No: OAGA00438

Size:100 ul

Sample(30 ug of whole cell lysate)
A:293T
12% SDS PAGE
OAGA00438 diluted at 1:1000

Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
OAGA00438 diluted at 1:10000

MDH2 antibody detects MDH2 protein by Western blot analysis.
A. 50 ug rat lung lysate/extract
10 % SDS-PAGE
MDH2 antibody (OAGA00438) dilution: 1:5000

MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MDH2 antibody (OAGA00438) dilution: 1:500.

MDH2 antibody detects MDH2 protein at mitochondrion on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MDH2 antibody (OAGA00438) dilution: 1:500.

MDH2 antibody detects MDH2 protein at mitochondrion on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
MDH2 antibody (OAGA00438) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
  Positive Controls: 293T, mouse brain, rat lung
  Cellular Localization: Mitochondrion matrix
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MDH2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Zebrafish (90%), Cat (95%), Pig (95%)
• Concentration: 0.76 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MDH2
• Gene Full Name: Malate dehydrogenase, mitochondrial
• Alias Symbols: MDH, MOR1, DEE51, M-MDH, EIEE51, MGC:3559
• NCBI Gene Id: 4191
• Protein Name: Malate dehydrogenase, mitochondrial
• Description of Target: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]
• Uniprot ID: P40926
• Protein Accession #: NP_005909.2
• Molecular Weight: 36 kDa