MDH2 Antibody - middle region (OAAB01682)

Catalog No: OAAB01682

Size:400ul

Western blot analysis of MDH2 Antibody (Center) in K562 cell line and mouse heart tissue lysates (35ug/lane). MDH2 (arrow) was detected using the purified Pab.(2ug/ml).

Western blot analysis of MDH2 (arrow) using rabbit polyclonal MDH2 Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the MDH2 gene.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Pig, Yeast
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the Central region of human MDH2.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Pig; Rat; Yeast
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: MDH2
• Alias Symbols: MDH, MOR1, DEE51, M-MDH, EIEE51, MGC:3559
• NCBI Gene Id: 4191
• Protein Name: Malate dehydrogenase, mitochondrial
• Uniprot ID: P40926
• Protein Accession #: NP_005909.2
• Molecular Weight: 35503 Da