MDH1 Antibody (OACA07214)

Catalog No: OACA07214

Immunohistochemistry of paraffin-embedded human skin tissue at dilution 1:100

Immunoprecipitating MDH1 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA03238 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA03238(4ug)+ HepG2 whole cell lysate(500ug)
Lane 3: HepG2 whole cell lysate (20ug)

All lanes: Malate dehydrogenase, cytoplasmic antibody at 4ug/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: mouse kidney tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 37,28,39 kDa
Observed band size: 36 kDa

Immunoprecipitating MDH1 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA03238 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA03238(4ug)+ HepG2 whole cell lysate(500ug)
Lane 3: HepG2 whole cell lysate (20ug)

All lanes: Malate dehydrogenase, cytoplasmic antibody at 4ug/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: mouse kidney tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 37,28,39 kDa
Observed band size: 36 kDa

Immunohistochemistry of paraffin-embedded human skin tissue at dilution 1:100

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014). [2]"Resveratrol-induced changes of the human adipocyte secretion profile." Rosenow A., Noben J.P., Jocken J., Kallendrusch S., Fischer-Posovszky P., Mariman E.C., Renes J.J. Proteome Res. 11:4733-4743(2012). [3]"Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity."Kim E.Y., Kim W.K., Kang H.J., Kim J.H., Chung S.J., Seo Y.S., Park S.G., Lee S.C., Bae K.H.J. Lipid Res. 53:1864-1876(2012).

Target Info

• Gene Symbol: MDH1
• Gene Full Name: malate dehydrogenase 1
• Alias Symbols: cytosolic malate dehydrogenase;DEE88;diiodophenylpyruvate reductase;EIEE88;epididymis secretory protein Li 32;HEL-S-32;KAR;malate dehydrogenase 1, NAD (soluble);malate dehydrogenase, cytoplasmic;malate dehydrogenase, peroxisomal;MDHA;MDH-s;MGC:1375;MOR2;soluble malate dehydrogenase.
• NCBI Gene Id: 4190
• Protein Name: Malate dehydrogenase, cytoplasmic
• Uniprot ID: P40925
• Protein Accession #: NP_001186040
• Nucleotide Accession #: NM_001199111