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| Datasheets/Manuals | Printable datasheet for anti-MDFI (ARP50514_P050) antibody |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human, Cow, Dog, Pig, Rabbit, Sheep |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of Human MDFI |
| Purification | Affinity purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 79%; Dog: 79%; Human: 100%; Pig: 86%; Rabbit: 79%; Sheep: 86% |
| Peptide Sequence | Synthetic peptide located within the following region: AVTCQPQGNPLGCTPLLPNDSGHPSELGGTRRAGNGALGGPKAHRKLQTH |
| Concentration | 0.5 mg/ml |
| Blocking Peptide | For anti-MDFI (ARP50514_P050) antibody is Catalog # AAP50514 |
| Reference | N/A |
| Gene Symbol | MDFI |
|---|---|
| Gene Full Name | MyoD family inhibitor |
| Alias Symbols | I-MF, I-mfa |
| NCBI Gene Id | 4188 |
| Protein Name | MyoD family inhibitor |
| Description of Target | This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. |
| Uniprot ID | Q99750 |
| Protein Accession # | NP_005577 |
| Protein Size (# AA) | 246 |
| Molecular Weight | 27kDa |
| Protein Interactions | PDIA5; USP20; ARID5A; ZNF266; KAT5; SPRY2; SPRY1; NR1H3; ZBTB24; ARHGAP32; KIAA0040; CREB5; MAGED1; ZNF264; GTF3C5; APLN; AKAP17A; ZNF230; ZNF223; ZNF155; ZNF138; ZNF136; ZNF124; ZNF79; WNT11; VHL; NR1H2; TYRO3; TNP2; TNNI1; TGIF1; GNAI2; GATA2; EIF4A2; D |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MDFI Antibody - middle region (ARP50514_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Cow, Dog, Pig, Rabbit, Sheep".
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How long will it take to receive "MDFI Antibody - middle region (ARP50514_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "MDFI Antibody - middle region (ARP50514_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MDFI Antibody - middle region (ARP50514_P050)"?
This target may also be called "I-MF, I-mfa" in publications.
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What is the shipping cost for "MDFI Antibody - middle region (ARP50514_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MDFI Antibody - middle region (ARP50514_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MDFI Antibody - middle region (ARP50514_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "27kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MDFI Antibody - middle region (ARP50514_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MDFI"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MDFI"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MDFI"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MDFI"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MDFI"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MDFI"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.