MDC1 Antibody (Phospho-Ser513) (OAAF00555)

Catalog No: OAAF00555 (Formerly GWB-ASB993)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MDC1 around the phosphorylation site of Ser513.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MDC1 (Phospho-Ser513) antibody detects endogenous levels of MDC1 only when phosphorylated at serine 513.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Nagase T., DNA Res. 3:17-24(1996).
  Shiina T., Genetics 173:1555-1570(2006).
  Mungall A.J., Nature 425:805-811(2003).

Target Info

• Gene Symbol: MDC1
• Gene Full Name: mediator of DNA damage checkpoint 1
• Alias Symbols: homologue to Drosophila photoreceptor protein calphotin;mediator of DNA damage checkpoint protein 1;NFBD1;nuclear factor with BRCT domains 1.
• NCBI Gene Id: 9656
• Protein Name: Mediator of DNA damage checkpoint protein 1
• Description of Target: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
• Uniprot ID: Q14676
• Protein Accession #: NP_055456.2
• Molecular Weight: 226 kDa