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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OASE00143-HRP |
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Predicted Species Reactivity | Human, Mouse, Chimpanzee, Bovine |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | P2B11 |
Isotype | IgG1 |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB, IF |
Additional Information | Background Info: Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus. |
:: | Scientific Background: MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139. |
:: | Certificate of Analysis: 0.5 ug/ml was sufficient for detection of MDC1 in 10ug of HeLa cell lysate by ECL immunoblot analysis. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | GST-tagged recombinant protein corresponding to mouse MDC1 ar and around the N-terminus |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:2000 (WB) |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781. |
Gene Symbol | Mdc1 |
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Gene Full Name | mediator of DNA damage checkpoint 1 |
Alias Symbols | NFBD, Nfbd1, AA413496, mKIAA0170, 6820401C03 |
NCBI Gene Id | 240087 |
Uniprot ID | Q5PSV9 |
Protein Accession # | NP_001010833.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MDC1 Antibody : HRP (OASE00143-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Chimpanzee, Bovine".
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How long will it take to receive "MDC1 Antibody : HRP (OASE00143-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "MDC1 Antibody : HRP (OASE00143-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MDC1 Antibody : HRP (OASE00143-HRP)"?
This target may also be called "NFBD, Nfbd1, AA413496, mKIAA0170, 6820401C03" in publications.
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What is the shipping cost for "MDC1 Antibody : HRP (OASE00143-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MDC1 Antibody : HRP (OASE00143-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MDC1 Antibody : HRP (OASE00143-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MDC1 Antibody : HRP (OASE00143-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MDC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MDC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MDC1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MDC1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MDC1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MDC1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.