MCT7 ELISA Kit (Pig) (OKCD02954)

Catalog No: OKCD02954

Size:96WELLS

Pig MCT7 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-98%	88-99%	92-102%	78-91%
  EDTA Plasma (n=5)	80-105%	93-105%	87-94%	78-89%
  Heparin Plasma (n=5)	80-89%	88-95%	79-93%	80-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase (TPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptase (TPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptase (TPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tryptase (TPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-101	85
  EDTA Plasma (n=5)	86-95	91
  Heparin Plasma(n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptase (TPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MCT7 Microplate: 96 Wells (12 x 8 Well strips)
  • MCT7 Lyophilized Standard: 2
  • 100X Biotinylated MCT7 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.123 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tryptase (TPS). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MCT7
• Gene Full Name: tryptase
• Alias Symbols: PMCT7;TPSB1;tryptase.
• NCBI Gene Id: 397389
• Protein Name: Tryptase
• Description of Target: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
• Uniprot ID: Q9N2D1
• Protein Accession #: NP_999356.1
• Nucleotide Accession #: NM_214191.1
• Protein Size (# AA): 275