MCPT4 ELISA Kit (Mouse) (OKWB00279)

Catalog No: OKWB00279

Size:96T

Mouse MCPT4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-104%	85-104%	85-99%	85-99%
  EDTA Plasma (n=5)	85-95%	87-93%	87-101%	82-101%
  Heparin Plasma (n=5)	81-98%	83-99%	80-93%	80-93%

• ELISA Kit Principle: Aviva Systems Biology Mcpt4 ELISA Kit (Mouse) (OKWB00279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mcpt4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mcpt4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mcpt4 captured in the well.
• ELISA Kit Range: 46.875-3000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-99	92
  EDTA Plasma (n=5)	87-96	91
  Heparin Plasma (n=5)	85-105	98

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Mcpt4 Microplate: 96 Wells (12 x 8 Well strips)
  • Mcpt4 Lyophilized Standard: 2 x 3 ng
  • 100X Mcpt4 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 28.1 pg/mL
• Publications:

  Nonlethal Plasmodium yoelii Infection Drives Complex Patterns of Th2-Type Host Immunity and Mast Cell-Dependent Bacteremia. Infect Immun. 88, NULL (2020). 32958528

• Assay Info: Assay Type: Quantitative Sandwich ELISA
• Description:
  View product citations for MCPT4 ELISA Kit (Mouse) (OKWB00279) on CiteAb

Target Info

• Gene Symbol: Mcpt4
• Gene Full Name: mast cell protease 4
• Alias Symbols: mast cell protease 4;Mcp;Mcp-;Mcp4;Mcp-4;MMCP-;MMCP-4;MMCP-4A;MMCP-4B;MSMCP;myo;myonase;serosal mast cell protease.
• NCBI Gene Id: 17227
• Protein Name: Mast cell protease 4
• Description of Target: Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.
• Uniprot ID: P21812
• Protein Accession #: NP_034909
• Nucleotide Accession #: NM_010779