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Catalog No: OCOA01626
Size:20UG
Price: $179.00
SKU
OCOA01626
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MCM7 Protein Lysate (OCOA01626)

Datasheets/ManualsPrintable datasheet for MCM7 Protein Lysate (OCOA01626)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMCM7 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMCM7
Gene Full Nameminichromosome maintenance complex component 7
Alias SymbolsCDC47;CDC47 homolog;DNA replication licensing factor MCM7;homolog of S. cerevisiae Cdc47;MCM2;minichromosome maintenance deficient 7;P1.1-MCM3;P1CDC47;P85MCM;PNAS146;PPP1R104;protein phosphatase 1, regulatory subunit 104.
NCBI Gene Id4176
Protein NameA0A0S2Z4A5, C6EMX8, NM_005916, NP_005907, P33993
Description of TargetDNA replication licensing factor MCM7, DNA replication licensing factor MCM7 isoform 1 [Homo sapiens], Homo sapiens minichromosome maintenance complex component 7 (MCM7), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P33993
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005907
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005916
Molecular Weight81.1 kDa
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