MCM3 ELISA Kit (Human) (OKCD00441)

Catalog No: OKCD00441 (Formerly GWB-KBBHW7)

Size:96WELLS

Human MCM3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	78-101%	80-94%	95-102%	82-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 3 (MCM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 3 (MCM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 3 (MCM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Minichromosome Maintenance Deficient 3 (MCM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	85-102	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 3 (MCM3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 3 (MCM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MCM3 Microplate: 96 Wells (12 x 8 Well Strips)
  • MCM3 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MCM3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 3 (MCM3). No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 3 (MCM3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MCM3
• Gene Full Name: minichromosome maintenance complex component 3
• Alias Symbols: cervical cancer proto-oncogene 5;DNA polymerase alpha holoenzyme-associated protein P1;DNA replication factor MCM3;DNA replication licensing factor MCM3;HCC5;hRlf beta subunit;MCM3 minichromosome maintenance deficient 3;minichromosome maintenance deficient 3;P1.h;p102;P1-MCM3;replication licensing factor, beta subunit;RLF subunit beta;RLFB.
• NCBI Gene Id: 4172
• Protein Name: DNA replication licensing factor MCM3
• Description of Target: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
• Uniprot ID: P25205
• Protein Accession #: NP_001257401
• Nucleotide Accession #: NM_001270472.1
• Protein Size (# AA): 808