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MCM3 Antibody (OAGA00887)

100 ul
$409.00
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Gene Symbol:
MCM3
Official Gene Full Name:
DNA replication licensing factor MCM3
NCBI Gene Id:
4172
Protein Name:
DNA replication licensing factor MCM3
Swissprot Id:
P25205
Protein Accession #:
NP_001257401.1
Alias Symbols:
HCC5 antibody, MGC1157 antibody, P1-MCM3 antibody, P1.h antibody, RLFB antibody, MCM3 antibody, RLF subunit beta antibody, MCM3 minichromosome maintenance deficient 3 antibody, minichromosome maintenance deficient 3 antibody, DNA replication factor MCM3 a
Replacement Item:
This antibody may replace item sc-173130, HPA004789
Molecular Weight:
91 kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Purified by antigen-affinity chromatography.
Application:
ICC, IF, IHC-FFPE, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MCM3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MCM3.
Immunogen:
Recombinant fragment corresponding to a region within amino acids 42 and 302 of MCM3 (Uniprot ID#P25205)
Predicted Species Reactivity:
Human
Product Format:
Liquid
Reconstitution and Storage:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-MCM3 antibody - OAGA00887
Isotype:
IgG
Application Info:
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Additional Information:
Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]
:::
Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
:::
Cellular Localization: Nucleus
Storage Buffer:
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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