Catalog No: OKEH04702
Size:96T
Price: $525.00
SKU
OKEH04702
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology MCM2 ELISA Kit (Human) (OKEH04702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MCM2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MCM2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MCM2 captured in well.
ELISA Kit Range0.078-5ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
MCM2 Microplate96 Wells (12 x 8 Well Strips)
MCM2 Standard2
Sample Diluent1 x 20 mL
MCM2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.039 ng/mL
SpecificityNatural and recombinant Human DNA replication licensing factor MCM2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMCM2
Gene Full Nameminichromosome maintenance complex component 2
Alias SymbolsBM28;CCNL1;cdc19;CDCL1;cell devision cycle-like 1;cyclin-like 1;D3S3194;DFNA70;DNA replication licensing factor MCM2;minichromosome maintenance deficient 2 (mitotin);minichromosome maintenance protein 2 homolog;MITOTIN;nuclear protein BM28.
NCBI Gene Id4171
Protein NameP49736
Description of TargetDNA replication licensing factor MCM2
Uniprot IDhttps://www.uniprot.org/uniprot/P49736
Protein Accession #NP_004517.2
Nucleotide Accession #NM_004526.3
Protein Size (# AA)904
Molecular Weight102 kDa
Write Your Own Review
You're reviewing:MCM2 ELISA Kit (Human) (OKEH04702)
Your Rating
We found other products you might like!