MCM2 Antibody (OAAF01861)

Catalog No: OAAF01861 (Formerly GWB-ASD132)

Size:100 ug

Western blot analysis of extracts from 293 cells, using MCM2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MCM2 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using MCM2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid PBS (without Mg²⁺ and Ca²⁺) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Isotype: IgG
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MCM2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using immunogen.
• Concentration: 1 mg/ml
• Specificity: MCM2 antibody detects endogenous levels of total MCM2 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1000
  IHC: 1:50~100
  IF: 1:100~500
  ELISA: 1:40000
• Reference: H Yan, Genes & Dev., Jun 1991; 5: 944 - 957.
  Yukio Ishimi, J. Biol. Chem., Nov 2001; 276: 42744 - 42752.
  Muluye E. Liku, Mol. Biol. Cell, Oct 2005; 16: 5026 - 5039.
  Daniela S. Dimitrova, J. Cell Biol., Aug 1999; 146: 709.

Target Info

• Gene Symbol: MCM2
• Gene Full Name: minichromosome maintenance complex component 2
• Alias Symbols: BM28;CCNL1;cdc19;CDCL1;cell devision cycle-like 1;cyclin-like 1;D3S3194;DFNA70;DNA replication licensing factor MCM2;minichromosome maintenance deficient 2 (mitotin);minichromosome maintenance protein 2 homolog;MITOTIN;nuclear protein BM28.
• NCBI Gene Id: 4171
• Protein Name: DNA replication licensing factor MCM2
• Description of Target: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
• Uniprot ID: P49736
• Protein Accession #: NP_004517.2
• Molecular Weight: 101 kDa