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Now Offering Over 102,157 Antibodies & 44,722 Antigens!

MCM2 antibody - N-terminal region (ARP36647_T100)

100 ul
$229.00
In Stock

Conjugation Options

ARP36647_T100-FITC Conjugated

ARP36647_T100-HRP Conjugated

ARP36647_T100-Biotin Conjugated

Gene Symbol:
MCM2
NCBI Gene Id:
4171
Official Gene Full Name:
Minichromosome maintenance complex component 2
Protein Name:
DNA replication licensing factor MCM2
Swissprot Id:
P49736
Protein Accession #:
NP_004517
Nucleotide Accession #:
NM_004526
Alias Symbols:
BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN
Replacement Item:
This antibody may replace item sc-10771 from Santa Cruz Biotechnology.
Description of Target:
The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Protein Size (# AA):
904
Molecular Weight:
99kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Protein A purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MCM2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MCM2.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human MCM2
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%
Complete computational species homology data:
Anti-MCM2 (ARP36647_T100)
Peptide Sequence:
Synthetic peptide located within the following region: PGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGM
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
HUWE1; MCM6; CHFR; SUMO2; MCMBP; UBC; SUMO1; MDM2; MCM7; MCM4; HSP90AB1; ACLY; PRRC1; HYOU1; CAP1; NCL; POLA1; TONSL; SNF8; ELL; TCEA1; POLR2A; CDK6; CDK4; CDC6; MCM10; ORC6; CCNB2; VCAM1; P2RX4; ORC5; ORC2; MCM5; MCM3; ITGA4; FN1; CSNK2A1; CDKN2A; DBF4;
Blocking Peptide:
For anti-MCM2 (ARP36647_T100) antibody is Catalog # AAP36647 (Previous Catalog # AAPP07904)
Datasheets/Manuals:
Printable datasheet for anti-MCM2 (ARP36647_T100) antibody
Sample Type Confirmation:

MCM2 is supported by BioGPS gene expression data to be expressed in Jurkat

Target Reference:
Yu,Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206
Publications:

Rizwani, W., Alexandrow, M. & Chellappan, S. Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication. Cell Cycle 8, 1621-9 (2009). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat 19377303

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1 Item(s)

02/01/2017 16:23
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Product Review: MCM2 antibody -N-terminal region (ARP36647_T100) in mouse neural stem cell lysate using Western blot
Product Page for MCM2 antibody-N-terminal region (ARP36647_T100)

Application: Western blotting
Researcher: Anonymous
Species + Tissue/Cell type: Lane 1: 5ug mouse neural stem cell lysate
Primary antibody dilution: 1:1000
Secondary antibody: Anti rabbit-IR-dye
Secondary antibody dilution: 1:10,000


Questionnaire:
How do Aviva's reagents play a role in your experimental goals? WB
How would you rate this antibody on a scale from 1-5 (5=best) nad why? 4
Would you use this antibody in future experiment? Yes
Have you used another antibody which has worked in your application? No
Do you believe the information about the reagent on Aviva's website is correct? Yes
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? May be. It is OK.
How did you store the antibody after re-suspension? Store aliquote @-20 degree C.
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): Mouse, adult neural stem cells lysate, 5ug.
How many different experimental trials were conducted using the antibody sample? 1
How was this sample prepared? Neural stem cells are harvested and lysed in 1X Laemmli buffer.
Primary antibody dilution and incubation time:  1:1000, Overnight.
Secondary antibody used and dilution and incubation time: Infrared dye-conjugated secondary antibody, 1:10000, 1hr.
Please include your detailed WB Procedure/Protocol here: 1. Protein sample was resolved on 10% SDS-PAGE;
2. Transfer proteins to Protran BA 85 nitrocellulose membranes (Whatman) by Semi-dry transfer;
3. Block the membrane for 1 hour at room temperature using 5% non-fat milk;
4. Incubate membrane with diluted primary antibody in 3% BSA solution overnight at 4 d C;
5. Wash the membrane in three washes of PBST, 5 minutes each;
6. Incubate the membrane with the diluted infrared dye-conjugated secondary antibody (Themo Scientific) in 5% non-fat milk in PBST at room temperature for 1 hour;
7. Wash the membrane in two washes of PBST, 5 minutes each, then wash in two washes of PBS.
8. Scan membrane in a Odyssey infrared imaging system.
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