MCL1 ELISA Kit (Mouse) (OKCD02718)

Catalog No: OKCD02718

Size:96WELLS

Mouse MCL1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-105%	78-90%	97-105%	95-102%
  EDTA Plasma (n=5)	92-105%	95-105%	84-104%	82-102%
  Heparin Plasma (n=5)	84-99%	98-105%	90-98%	80-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-95	91
  EDTA Plasma (n=5)	95-104	98
  Heparin Plasma(n=5)	91-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MCL1 Microplate: 96 Wells (12 x 8 Well strips)
  • MCL1 Lyophilized Standard: 2
  • 100X Biotinylated MCL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mcl1
• Gene Full Name: myeloid cell leukemia sequence 1
• Alias Symbols: AW556805;bcl-2-related protein EAT/mcl1;induced myeloid leukemia cell differentiation protein Mcl-1 homolog;Mcl-;Mcl-1.
• NCBI Gene Id: 17210
• Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
• Description of Target: Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity.
• Uniprot ID: P97287
• Protein Accession #: NP_032588.1
• Nucleotide Accession #: NM_008562.3
• Protein Size (# AA): 331