MCAM Chemi-Luminescent ELISA Kit (Mouse) (OKCD03963)

Catalog No: OKCD03963

Size:96WELLS

Mouse MCAM ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-101%	91-99%	93-101%	90-97%
  EDTA Plasma (n=5)	87-97%	83-90%	80-91%	80-94%
  Heparin Plasma (n=5)	93-101%	78-92%	97-105%	91-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 146 (CD146). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 146 (CD146). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cluster Of Differentiation 146 (CD146) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	101
  EDTA Plasma (n=5)	89-101	94
  Heparin Plasma (n=5)	81-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 146 (CD146) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 146 (CD146) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mcam Microplate: 96 Wells (12 x 8 Well strips)
  • Mcam Lyophilized Standard: 2
  • 100X Mcam Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.625 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mcam
• Gene Full Name: melanoma cell adhesion molecule
• Alias Symbols: 1-gic;1-gicerin;AV025631;CD146;CD149;cell surface glycoprotein MUC18;gicerin;l-gicerin protein;melanoma adhesion molecule;Melanoma cell adhesion molecule;melanoma-associated antigen MUC18;Muc18;s-e;s-endo;s-gi;s-gicerin.
• NCBI Gene Id: 84004
• Protein Name: Cell surface glycoprotein MUC18
• Description of Target: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).
• Uniprot ID: Q8R2Y2
• Protein Accession #: NP_075548
• Nucleotide Accession #: NM_023061