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MC2R ELISA Kit (Guinea pig) (OKCA01050)

96 Wells
$675.00
In Stock
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Gene Symbol:
MC2R
Official Gene Full Name:
Adrenocorticotropic hormone receptor
NCBI Gene Id:
100135574
Alias Symbols:
ACTHR, MGC125798, ACTH receptor|MC2 receptor|OTTHUMP00000162512|corticotropin receptor|melanocortin 2 receptor
Description of Target:
Receptor for corticotropin (ACTH). This receptor is mediated by G proteins (G(s)) which activate adenylate cyclase (cAMP).
Protein Name:
Adrenocorticotropic hormone receptor
Swissprot Id:
Q9Z1S9
Protein Accession #:
NP_001166453.1
Nucleotide Accession #:
NM_001172982.1
Predicted Species Reactivity:
Guinea Pig
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
3.9 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01050 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01050 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology MC2R ELISA Kit (Guinea pig) (OKCA01050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MC2R has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MC2R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MC2R captured in the well.
Kit Component:
ComponentAmount
MC2R Microplate96 Wells (12 x 8 Well strips)
MC2R Lyophilized Standard2 x 1 ng
100X Biotinylated MC2R Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat9389-98
1:29691-100
1:48682-90
1:8105100-110
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9590-100
EDTA Plasma (n=4)8984-93
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MC2R.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MC2R.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA

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