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MBP Polyclonal Antibody (OABB00147)
Datasheets/Manuals | Printable datasheet for MBP Polyclonal Antibody (OABB00147) |
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Tested Species Reactivity | Human, Mouse, Rat |
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Predicted Species Reactivity | Hamster|Human|Mouse|Rat |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Immunofluorescence|Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Dilution | Optimal dilutions should be determined by end user. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse, Rat: Human: By Heat Immunofluorescence: 2 ug/ml: Mouse, Rat |
Reference | 1. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D, Pessac B.The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc Natl Acad Sci U S A. 2002 Jun 25; 99(13):8856-61. 2. Streicher R, Stoffel W.The organization of the human myelin basic protein gene. Comparison with the mouse gene. Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10. 3. McLaurin JA, Hafler DA, Antel JP.Reactivity of normal T-cell lines to MBP isolated from normal and multiple sclerosis white matter. J Neurol Sci. 1995 Feb; 128(2):205-11. 4. Iester A, Vignola S, Callegarini L, Gimelli G, Alpigiani MG. [18q syndrome with deficiency of myelin basic protein (MBP)] Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5. 5. Tranquill LR, Skinner E, Campagnoni C, Vergelli M, Hemmer B, Muraro P, Martin R, McFarland HF, Campagnoni AT, Voskuhl RR. Human T lymphocytes specific for the immunodominant 83-99 epitope of myelin basic protein: recognition of golli MBP HOG 7. J Neurosci Res. 1996 Sep 15; 45(6):820-8. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Publications | Cerebral Apolipoprotein-D Is Hypoglycosylated Compared to Peripheral Tissues and Is Variably Expressed in Mouse and Human Brain Regions. PLoS ONE. 11, e0148238 (2016). 26829325 |
Description |
Gene Symbol | MBP |
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Gene Full Name | myelin basic protein |
Alias Symbols | Golli-MBP;myelin A1 protein;myelin basic protein;myelin membrane encephalitogenic protein;proline-rich proteoglycan 2. |
NCBI Gene Id | 4155 |
Protein Name | Myelin basic protein |
Description of Target | The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. |
Uniprot ID | P02686 |
Molecular Weight | 33117 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MBP Polyclonal Antibody (OABB00147)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Hamster|Human|Mouse|Rat".
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How long will it take to receive "MBP Polyclonal Antibody (OABB00147)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "MBP Polyclonal Antibody (OABB00147)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MBP Polyclonal Antibody (OABB00147)"?
This target may also be called "Golli-MBP;myelin A1 protein;myelin basic protein;myelin membrane encephalitogenic protein;proline-rich proteoglycan 2." in publications.
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What is the shipping cost for "MBP Polyclonal Antibody (OABB00147)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MBP Polyclonal Antibody (OABB00147)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MBP Polyclonal Antibody (OABB00147)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "33117 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MBP Polyclonal Antibody (OABB00147)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MBP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MBP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MBP"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MBP"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MBP"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MBP"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.