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Catalog No: OKCA02242
Size:96 Wells
Price: $929.00
SKU
OKCA02242
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)Neat9087-93
Serum (n=4)1:29995-103
Serum (n=4)1:49288-96
Serum (n=4)1:89591-100
ELISA Kit PrincipleAviva Systems Biology MBP ELISA Kit (Rat) (OKCA02242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MBP captured in well.
ELISA Kit Range0.312 ng/mL-20 ng/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)9085-96
EDTA plasma (n=4)9592-97
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-MBP Microplate96 Wells (12 x 8 Well strips)
MBP Lyophilized Standard 2 x 20 ng
100X Biotinylated MBP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMBP
Protein NameMyelin basic protein
Description of TargetIs, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
Uniprot IDP25274
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