Catalog No: OKEH00505
Size:96T
Price: $525.00
SKU
OKEH00505
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MBP ELISA Kit (Human) : 96 Wells (OKEH00505)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00505
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MBP ELISA Kit (Human) (OKEH00505) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human MBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MBP captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.2% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MBP Microplate96 Wells (12 x 8 Well strips)
MBP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X MBP-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.21 ng/mL
SpecificityNatural and recombinant Human Myelin basic protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMBP
Gene Full Namemyelin basic protein
Alias SymbolsGolli-MBP;myelin A1 protein;myelin basic protein;myelin membrane encephalitogenic protein;proline-rich proteoglycan 2.
NCBI Gene Id4155
Protein NameP02686
Description of TargetMyelin basic protein
Uniprot IDhttps://www.uniprot.org/uniprot/P02686
Protein Accession #NP_001020252.1
Nucleotide Accession #NM_001025081.1
Protein Size (# AA)304
Molecular Weight33 kDa
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