MBNL1 Antibody - middle region (ARP41227_P050)

Catalog#: ARP41227_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: MBNL1
• Official Gene Full Name: Muscleblind-like splicing regulator 1
• NCBI Gene Id: 4154
• Protein Name: MBNL1 protein EMBL AAH50535.1
• Swissprot Id: Q86VM6
• Protein Accession #: AAH50535
• Nucleotide Accession #: NM_021038
• Alias Symbols: EXP, MBNL, EXP35, EXP40, EXP42
• Replacement Item: This antibody may replace item sc-115973 from Santa Cruz Biotechnology.
• Description of Target: MBNL1 contains 4 C3H1-type zinc fingers and binds to CUG triplet repeat expansion dsRNA.
• Protein Size (# AA): 343
• Molecular Weight: 38kDa
• Purification: Affinity Purified
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express MBNL1.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MBNL1
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Horse: 100%; Human: 100%; Mouse: 79%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: AMTQSAVKSLKRPLEATFDLGIPQAVLPPLPKRPALEKTNGATAVFNTGI
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: DAB1; AES; AEN; UBC; ABI3BP; ZNHIT3; VCAM1; ITGA4; IDH3A; FN1; TIAL1; APP; BIN1; ITSN2; DDX17; HNRNPL; HNRNPK; HNRNPH3; HNRNPH2; HNRNPH1; HNRNPF; HNRNPA2B1; DHX9; DDX5;
• Blocking Peptide: For anti-MBNL1 (ARP41227_P050) antibody is Catalog # AAP41227 (Previous Catalog # AAPS01306)
• Sample Type Confirmation:

  MBNL1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Target Reference: Strausberg,R., (2003) Direct Submission
• Publications:

  Coram, RJ; Stillwagon, SJ; Guggilam, A; Jenkins, MW; Swanson, MS; Ladd, AN; Muscleblind-like 1 is required for normal heart valve development in vivo. 15, 36 (2015). IHC, WB, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish 26472242

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: MBNL1 antibody tested with Human Jurkat Cells (ARP41227_P050)

Product Protocols: MBNL1 antibody tested by IHC with human heart (ARP41227)