MBL2 ELISA Kit (Rat) (OKEH04472)

Catalog No: OKEH04472

Size:96T

Rat MBL2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Mbl2 ELISA Kit (Rat) (OKEH04472) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mbl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mbl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mbl2 captured in the well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Component:
  • Anti-Mbl2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mbl2 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Mbl2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39.25 pg/mL
• Specificity: Natural and recombinant Rat Mannose-binding protein C
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LOC100911854, Mbl2
• Gene Full Name: mannose binding lectin 2, mannose-binding protein C-like
• Alias Symbols: Ab2-001;Ab2-011;mannan-binding protein;mannose binding lectin 2 (protein C);mannose-binding lectin (protein C) 2;mannose-binding protein C;mannose-binding protein C (liver);mannose-binding protein C-like;MBP-C;RA-reactive factor P28A subunit;ra-reactive factor polysaccharide-binding component p28A;raRF p28A;RARF/P28A.
• NCBI Gene Id: 100911854, 64668
• Protein Name: P08661
• Description of Target: Mannose-binding protein C
• Uniprot ID: https://www.uniprot.org/uniprot/P08661
• Protein Accession #: NP_073195.2
• Nucleotide Accession #: NM_022704.2
• Protein Size (# AA): 244
• Molecular Weight: 26 KDa