Catalog No: OCOA10528
Size:20UG
Price: $179.00
SKU
OCOA10528
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MBD1 Protein Lysate (OCOA10528)

Datasheets/ManualsPrintable datasheet for MBD1 Protein Lysate (OCOA10528)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMBD1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMBD1
Gene Full Namemethyl-CpG binding domain protein 1
Alias SymbolsCXXC3;CXXC-type zinc finger protein 3;methyl-CpG-binding domain protein 1;Methyl-CpG-binding protein MBD1;PCM1;protein containing methyl-CpG-binding domain 1;RFT;the regulator of fibroblast growth factor 2 (FGF-2) transcription.
NCBI Gene Id4152
Protein NameNM_015844, NP_056669, Q9UIS9
Description of TargetHomo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, mRNA, Methyl-CpG-binding domain protein 1, methyl-CpG-binding domain protein 1 isoform 3 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UIS9
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_056669
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_015844
Molecular Weight59.8 kDa
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