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Catalog No: OCOA09074
Size:20UG
Price: $179.00
SKU
OCOA09074
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MAVS Protein Lysate (OCOA09074)

Datasheets/ManualsPrintable datasheet for MAVS Protein Lysate (OCOA09074)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMAVS HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMAVS
Gene Full Namemitochondrial antiviral signaling protein
Alias SymbolsCARD adapter inducing interferon beta;CARD adaptor inducing IFN-beta;CARDIF;IFN-B promoter stimulator 1;interferon beta promoter stimulator protein 1;IPS1;IPS-1;mitochondrial antiviral-signaling protein;putative NF-kappa-B-activating protein 031N;virus-induced signaling adaptor;virus-induced-signaling adapter;VISA.
NCBI Gene Id57506
Protein NameNM_020746, NP_065797, Q7Z434
Description of TargetHomo sapiens mitochondrial antiviral signaling protein (MAVS), transcript variant 1, mRNA, Mitochondrial antiviral-signaling protein, mitochondrial antiviral-signaling protein isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q7Z434
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_065797
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_020746
Molecular Weight56.6 kDa
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