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MATR3 ELISA Kit (Human) (OKCD01966)

96 Wells
$599.00
In Stock
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Predicted Species Reactivity:
Human
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~3.5 Hours
Kit Principle:
Aviva Systems Biology MATR3 ELISA Kit (Human) (OKCD01966) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MATR3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MATR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MATR3 captured in the well.
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level MATR3 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level MATR3 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Component:
ComponentAmount
Anti-MATR3 Microplate96 Wells (12 x 8 Well Strips)
MATR3 Lyophilized Standard2 x 20 ng
100X Biotinylated MATR3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Tissue homogenates and other biological fluids
Sensitivity:
< 0.117 ng/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Matrin 3 (MATR3). No significant cross-reactivity or interference between Matrin 3 (MATR3) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Gene Symbol:
MATR3
Official Gene Full Name:
Matrin 3
Alias Symbols:
DKFZp686K0542, DKFZp686K23100, KIAA0723, Matrin-3, MGC9105, MPD2, VCPDM
NCBI Gene Id:
9782
Protein Name:
Matrin-3
Description of Target:
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
Swissprot Id:
P43243
Protein Accession #:
NP_001181883.1
Nucleotide Accession #:
NM_001194954.1
Protein Size (# AA):
847
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MATR3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MATR3.

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