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Catalog No: OKEH02254
Size:96T
Price: $525.00
SKU
OKEH02254
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MAT2A ELISA Kit (Human) (OKEH02254) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAT2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAT2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAT2A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 10.3% (n = 20)
ELISA Kit Component
ComponentAmount
MAT2A Microplate96 Wells (12 x 8 Well strips)
MAT2A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MAT2A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.15 ng/mL
SpecificityNatural and recombinant Human S-adenosylmethionine synthase isoform type-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMAT2A
Gene Full Namemethionine adenosyltransferase 2A
Alias SymbolsadoMet synthase 2;adoMet synthetase 2;MAT 2;MATA2;MATII;MAT-II;methionine adenosyltransferase 2;Methionine adenosyltransferase II;methionine adenosyltransferase II, alpha;S-adenosylmethionine synthase isoform type-2;SAMS2;testicular tissue protein Li 121.
NCBI Gene Id4144
Protein NameP31153
Description of TargetS-adenosylmethionine synthase isoform type-2
Uniprot IDhttps://www.uniprot.org/uniprot/P31153
Protein Accession #NP_005902.1
Nucleotide Accession #NM_005911.5
Protein Size (# AA)395
Molecular Weight44 kDa
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