Catalog No: OCOA00143
Size:20UG
Price: $179.00
SKU
OCOA00143
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MAT1A Protein Lysate (OCOA00143)

Datasheets/ManualsPrintable datasheet for MAT1A Protein Lysate (OCOA00143)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMAT1A HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMAT1A
Gene Full Namemethionine adenosyltransferase 1A
Alias SymbolsadoMet synthase 1;adoMet synthetase 1;MAT;MAT 1;MATA1;MAT-I/III;methionine adenosyltransferase 1;methionine adenosyltransferase I, alpha;methionine adenosyltransferase I/III;S-adenosylmethionine synthase isoform type-1;SAMS;SAMS1.
NCBI Gene Id4143
Protein NameNM_000429, NP_000420, Q00266
Description of TargetHomo sapiens methionine adenosyltransferase 1A (MAT1A), mRNA, S-adenosylmethionine synthase isoform type-1, S-adenosylmethionine synthase isoform type-1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q00266
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000420
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000429
Molecular Weight43.6 kDa
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