MAT1A ELISA Kit (Rat) (OKCD08941)

Catalog No: OKCD08941

Size:96WELLS

MAT1A ELISA Kit (Rat) (OKCD08941) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase I Alpha (MAT1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase I Alpha (MAT1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase I Alpha (MAT1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Methionine Adenosyltransferase I Alpha (MAT1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.781-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase I Alpha (MAT1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase I Alpha (MAT1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MAT1A Microplate: 96 Wells (12 x 8 Well strips)
  • MAT1A Lyophilized Standard: 2
  • 100X MAT1A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.32ng/mL

Target Info

• Gene Symbol: Mat1a
• Gene Full Name: methionine adenosyltransferase 1A
• Alias Symbols: AdoMet;adoMet synthase 1;adoMet synthetase 1;MAT 1;MAT-I/III;methionine adenosyltransferase 1;methionine adenosyltransferase I, alpha;methionine adenosyltransferase I/III;S - adenosylmethionine synthetase;SADE;S-adenosylmethionine synthase isoform type-1;SAS.
• NCBI Gene Id: 25331
• Protein Name: S-adenosylmethionine synthase isoform type-1
• Description of Target: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
• Uniprot ID: P13444
• Protein Accession #: NP_036992.2
• Nucleotide Accession #: NM_012860.2