MAT1A Chemi-Luminescent ELISA Kit (Rat) (OKCD03733)

Catalog No: OKCD03733

Size:96WELLS

Rat MAT1A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase I Alpha (MAT1a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase I Alpha (MAT1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Methionine Adenosyltransferase I Alpha (MAT1a) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase I Alpha (MAT1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase I Alpha (MAT1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mat1a Microplate: 96 Wells (12 x 8 Well strips)
  • Mat1a Lyophilized Standard: 2
  • 100X Mat1a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mat1a
• Gene Full Name: methionine adenosyltransferase 1A
• Alias Symbols: AdoMet;adoMet synthase 1;adoMet synthetase 1;MAT 1;MAT-I/III;methionine adenosyltransferase 1;methionine adenosyltransferase I, alpha;methionine adenosyltransferase I/III;S - adenosylmethionine synthetase;SADE;S-adenosylmethionine synthase isoform type-1;SAS.
• NCBI Gene Id: 25331
• Protein Name: S-adenosylmethionine synthase isoform type-1
• Description of Target: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
• Uniprot ID: P13444
• Protein Accession #: NP_036992
• Nucleotide Accession #: NM_012860